大鼠肿瘤坏死因子α(TNFa)（用于细胞上清）酶联免疫吸附检测试剂盒

英文名称	Rat TNFa(Tumor Necrosis Factor Alpha)(For cellular supernatant) ELISA Kit
别名	TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

种属	Rat
检测类型	Sandwich
灵敏度	6.3 pg/mL
检测范围	15.63-1000 pg/mL
样本类型	Cell culture supernates,cell lysates,tissue homogenates
实验时长	3.5h

研究领域	Cytokine;Tumor immunity;Infection immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P16599

标准曲线图

【仅供科学研究使用】

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