小鼠核因子κB亚基p105(NF-κB p105)酶联免疫吸附检测试剂盒
- 货号：ELK1337
- 价格：￥2,400.00 元
- 点击：1812
产品介绍
产品信息
| 英文名称 | Mouse NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit |
|---|
| 别名 | NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B |
|---|
规格与价格
| 48T 价格 | 1680 |
|---|
| 96T 价格 | 2400 |
|---|
| 96T*5 价格 | 10200 |
|---|
检测参数
| 种属 | Mouse |
|---|
| 检测类型 | Sandwich |
|---|
| 灵敏度 | 0.058 ng/mL |
|---|
| 检测范围 | 0.16-10 ng/mL |
|---|
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
|---|
| 实验时长 | 3.5h |
|---|
研究信息
| 研究领域 | Signal transduction; |
|---|
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
| UniProt编号 | P25799 |
|---|
标准曲线
| 标准曲线图 |  |
|---|
