人白介素18(IL18)酶联免疫吸附检测试剂盒

英文名称	Human IL18(Interleukin 18) ELISA Kit
别名	IL-18；IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

种属	Human
检测类型	Sandwich
灵敏度	37.2 pg/mL
检测范围	125-8000 pg/mL
样本类型	serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Cytokine;Infection immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL18 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q14116

标准曲线图

【仅供科学研究使用】

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