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豚鼠肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒

  • 货号:ELK1215
  • 价格:¥2,400.00 元
  • 点击:672

产品介绍

产品信息

英文名称Guinea pig TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
别名TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Guinea pig
检测类型Sandwich
灵敏度2.9 pg/mL
检测范围7.82-500 pg/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cytokine;Tumor immunity;Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P51435

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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