大鼠5-羟色胺受体1A(5HTR1A)酶联免疫吸附检测试剂盒

英文名称	Rat 5HTR1A(5-Hydroxytryptamine Receptor 1A) ELISA Kit
别名	5HTR1A; 5HTR1-A; 5-HTR1-A; ADRB2RL1; ADRBRL1; Serotonin Receptor 1A

种属	Rat
检测类型	Sandwich
灵敏度	31.6 pg/mL
检测范围	78.13-5000 pg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Neuro science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat 5HTR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 5HTR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat 5HTR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 5HTR1A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P19327

标准曲线图

【仅供科学研究使用】

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