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绵羊热休克蛋白70(HSP-70/HSPA9)酶联免疫吸附检测试剂盒

  • 货号:ELK11297
  • 价格:¥2,800.00 元
  • 点击:2192

产品介绍

产品信息

英文名称Sheep HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit
别名DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Sheep
检测类型Sandwich
灵敏度1.34 ng/mL
检测范围3.13-200 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Tumor immunity;Infection immunity;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep HSP-70. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep HSP-70. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep HSP-70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep HSP-70 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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