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花生四烯酸(AA)酶联免疫吸附检测试剂盒

  • 货号:ELK11240
  • 价格:¥2,800.00 元
  • 点击:1714

产品介绍

产品信息

英文名称AA(Arachidonic Acid) ELISA Kit
别名ARA

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属General
检测类型Competitive Inhibition
灵敏度0.57 ng/mL
检测范围1.57-100 ng/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长2h

研究信息

研究领域Signal transduction;Metabolic pathway;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;
检测原理This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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