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兔可溶性末端补体复合物sC5b-9(sC5b-9)酶联免疫吸附检测试剂盒

  • 货号:ELK11213
  • 价格:¥2,800.00 元
  • 点击:2056

产品介绍

产品信息

英文名称Rabbit sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
别名MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rabbit
检测类型Sandwich
灵敏度12.8 ng/mL
检测范围31.25-2000 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Immunology;Metabolism;Signal Transduction
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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