大鼠转铁蛋白受体(TFR)酶联免疫吸附检测试剂盒

英文名称	Rat TFR(Transferrin Receptor) ELISA Kit
别名	CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

种属	Rat
检测类型	Sandwich
灵敏度	0.053 µg/mL
检测范围	0.16-10 µg/mL
样本类型	Serum, plasma and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q99376

标准曲线图

【仅供科学研究使用】

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