大鼠α1-抗胰蛋白酶(a1AT)微量上样酶联免疫吸附检测试剂盒

英文名称	Rat a1AT(Alpha-1-Antitrypsin) Microsample ELISA Kit
别名	SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

种属	Rat
检测类型	Sandwich
灵敏度	4.93 µg/mL
检测范围	15.63-1000 µg/mL
样本类型	Serum, plasma and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;Infection immunity;Hepatology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat a1AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat a1AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat a1AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat a1AT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P17475

标准曲线图

【仅供科学研究使用】

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