大鼠胰岛素样生长因子结合蛋白3(IGFBP3)酶联免疫吸附检测试剂盒

英文名称	Rat IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
别名	BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

种属	Rat
检测类型	Sandwich
灵敏度	0.133 µg/mL
检测范围	0.312-20 µg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Metabolic pathway;Tumor immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P15473

标准曲线图

【仅供科学研究使用】

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