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小鼠纤溶酶-抗纤溶酶复合物(PAP)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK11018MS
  • 价格:¥2,400.00 元
  • 点击:2149

产品介绍

产品信息

英文名称Mouse PAP(Plasmin-Antiplasmin Complex) Microsample ELISA Kit
别名PAP

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Mouse
检测类型Sandwich
灵敏度2 ng/mL
检测范围7.81-500 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cardiovascular;Cancer;Cell Biology
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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