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大鼠爱帕琳肽13(AP13)酶联免疫吸附检测试剂盒

  • 货号:ELK10782
  • 价格:¥2,800.00 元
  • 点击:2015

产品介绍

产品信息

英文名称Rat AP13(Apelin 13) ELISA Kit
别名APLN13

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Competitive Inhibition
灵敏度0.23 ng/mL
检测范围0.78-50 ng/mL
样本类型Serum, plasma and other biological fluids
实验时长2h

研究信息

研究领域Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;
检测原理This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AP13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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