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大鼠脑源性神经营养因子(BDNF)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK10643MS
  • 价格:¥2,400.00 元
  • 点击:1431

产品介绍

产品信息

英文名称Rat BDNF(Brain Derived Neurotrophic Factor) Microsample ELISA Kit
别名Neurotrophin; Abrineurin

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Rat
检测类型Sandwich
灵敏度0.111 ng/mL
检测范围0.32-20 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cytokine;Infection immunity;Neuro science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BDNF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P23363

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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