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大鼠基质金属蛋白酶9(MMP9)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK10458MS
  • 价格:¥2,600.00 元
  • 点击:1226

产品介绍

产品信息

英文名称Rat MMP9(Matrix Metalloproteinase 9) Microsample ELISA Kit
别名GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度1.8 ng/mL
检测范围7.81-500 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MMP9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MMP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MMP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P50282

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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