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小鼠Ⅰ型胶原α2(COL1a2)酶联免疫吸附检测试剂盒

  • 货号:ELK10346
  • 价格:¥2,600.00 元
  • 点击:1536

产品介绍

产品信息

英文名称Mouse COL1a2(Collagen Type I Alpha 2) ELISA Kit
别名COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.23 ng/mL
检测范围0.78-50 ng/mL
样本类型Serum, plasma and other biological fluids
实验时长3.5h

研究信息

研究领域Metabolic pathway;Bone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1a2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL1a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL1a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL1a2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q01149

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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