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大鼠血小板反应蛋白1(TSP-1)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK10312MS
  • 价格:¥2,600.00 元
  • 点击:1044

产品介绍

产品信息

英文名称Rat TSP-1(Thrombospondin-1) Microsample ELISA Kit
别名TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度0.47 ng/mL
检测范围1.56-100 ng/mL
样本类型Plasma
实验时长3.5h

研究信息

研究领域Infection immunity;Hematology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q71SA3

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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