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大鼠血管紧张素Ⅱ(AngII)酶联免疫吸附检测试剂盒

  • 货号:ELK10264
  • 价格:¥2,600.00 元
  • 点击:2098

产品介绍

产品信息

英文名称Rat AngII(Angiotensin II) ELISA Kit
别名Ang-II; Angiotensin-2

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度9.95 pg/mL
检测范围31.25-2000 pg/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Endocrinology;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AngII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AngII in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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