大鼠高敏C反应蛋白(hs-CRP)酶联免疫吸附检测试剂盒

英文名称	Rat hs-CRP(High Sensitivity C Reactive Protein) ELISA Kit
别名	C-RP; PTX1; Pentraxin-Related; Pentraxin 1

种属	Rat
检测类型	Sandwich
灵敏度	0.16 µg/mL
检测范围	0.78-50 µg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P48199

标准曲线图

【仅供科学研究使用】

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