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人嗜酸性粒细胞阳离子蛋白(ECP)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK10036MS
  • 价格:¥2,600.00 元
  • 点击:1767

产品介绍

产品信息

英文名称Human ECP(Eosinophil Cationic Protein) Microsample ELISA Kit
别名Ribonuclease 3; RNase 3

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度1.54 ng/mL
检测范围3.13-200 ng/mL
样本类型serum, plasma and other biological fluids
实验时长3.5h

研究信息

研究领域Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ECP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ECP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P12724

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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