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大鼠硫氧还蛋白互作蛋白(TXNIP)酶联免疫吸附检测试剂盒

  • 货号:ELK0998
  • 价格:¥2,600.00 元
  • 点击:2891
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产品介绍

产品信息

英文名称Rat TXNIP(Thioredoxin-interacting protein) ELISA Kit
别名TXNIP; VDUP1; Thioredoxin-interacting protein; Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度4.4 pg/mL
检测范围15.625-1000 pg/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Metabolic pathway;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TXNIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TXNIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TXNIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TXNIP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q5M7W1

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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