大鼠磷酸化腺苷酸活化蛋白激酶(p-AMPK)酶联免疫吸附检测试剂盒
- 货号：ELK0962
- 价格：￥2,600.00 元
- 点击：1338
产品介绍
产品信息
| 英文名称 | Rat p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit |
|---|
| 别名 | p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1 |
|---|
规格与价格
| 48T 价格 | 1820 |
|---|
| 96T 价格 | 2600 |
|---|
| 96T*5 价格 | 11050 |
|---|
检测参数
| 种属 | Rat |
|---|
| 检测类型 | Sandwich |
|---|
| 灵敏度 | 1.25 ng/mL |
|---|
| 检测范围 | 3.13-200 ng/mL |
|---|
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
|---|
| 实验时长 | 3.5h |
|---|
研究信息
| 研究领域 | Enzyme & Kinase |
|---|
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
标准曲线
| 标准曲线图 |  |
|---|
