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大鼠磷酸化c-Jun氨基末端激酶(p-JNK)酶联免疫吸附检测试剂盒

  • 货号:ELK0960
  • 价格:¥2,800.00 元
  • 点击:702

产品介绍

产品信息

英文名称Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit
别名Bsk; BSK; CG5680; D-JNK; D-junk; DBSK/JNK; dJnk; dJNK; DJNK; DJNK/bsk; Dmel\CG5680; DmJNK; dNJNK; jnk; Jnk; JNK; jnk (bsk); JNK/SAPK; Junk; p-JNK; P-JNK; pJNK; SAPKa

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.148 ng/mL
检测范围0.32-20 ng/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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