人S-腺苷蛋氨酸(SAM)酶联免疫吸附检测试剂盒

英文名称	Human SAM(S-Adenosyl Methionine) ELISA Kit
别名	SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride

种属	Human
检测类型	Competitive Inhibition
灵敏度	5.8 ng/mL
检测范围	15.63-1000 ng/mL
样本类型	serum, plasma, cell lysates, cell culture supernates and other biological fluids
实验时长	2h

研究领域	Enzyme & Kinase;
检测原理	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P17707

标准曲线图

【仅供科学研究使用】

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