人磷酸化AKT(S473)酶联免疫吸附检测试剂盒
- 货号：ELK0792
- 价格：￥2,600.00 元
- 点击：2072
产品介绍
产品信息
| 英文名称 | Human Phospho AKT S473 (p-AKT) ELISA Kit |
|---|---|
| 别名 | p-AKT |
规格与价格
| 48T 价格 | 1820 |
|---|---|
| 96T 价格 | 2600 |
| 96T*5 价格 | 11050 |
检测参数
| 种属 | Human |
|---|---|
| 检测类型 | Sandwich |
| 灵敏度 | 52.5 pg/mL |
| 检测范围 | 156.25-10000 pg/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 实验时长 | 3.5h |
研究信息
| 研究领域 | Enzyme & Kinase; |
|---|---|
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve. |
标准曲线
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