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一步法人胰岛素样生长因子结合蛋白3(IGFBP3)酶联免疫吸附检测试剂盒

  • 货号:ELK059ES
  • 价格:¥2,600.00 元
  • 点击:2011

产品介绍

产品信息

英文名称EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
别名BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.98 pg/mL
检测范围78.13-5000 pg/mL
样本类型Serum, plasma
实验时长1.5h

研究信息

研究领域Metabolic pathway;Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3, and the Human IGFBP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP3. After TMB substrate solution is added, only those wells that contain Human IGFBP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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