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一步法大鼠游离三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒

  • 货号:ELK050ES
  • 价格:¥2,800.00 元
  • 点击:2053

产品介绍

产品信息

英文名称EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit
别名3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Competitive Inhibition
灵敏度0.31 ng/mL
检测范围0.32-20 ng/mL
样本类型Serum, plasma
实验时长1.5h

研究信息

研究领域Endocrinology;Autoimmunity;
检测原理This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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