人Ⅴ型胶原α1(COL5a1)微量上样酶联免疫吸附检测试剂盒

英文名称	Human COL5a1(Collagen Type V Alpha 1) Microsample ELISA Kit
别名	AB collagen; Alpha 1 type V collagen; COL5A1; Collagen alpha 1(V) chain

种属	Human
检测类型	Sandwich
灵敏度	0.061 ng/mL
检测范围	0.16-10 ng/mL
样本类型	serum, plasma, tissue homogenates and other biological fluids
实验时长	3.5h

研究领域	Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL5a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL5a1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P20908

标准曲线图

【仅供科学研究使用】

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