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人压电型机械敏感离子通道组件1(PIEZO-1)酶联免疫吸附检测试剂盒

  • 货号:ELK0343
  • 价格:¥2,600.00 元
  • 点击:1519

产品介绍

产品信息

英文名称Human PIEZO-1(Piezo-type mechanosensitive ion channel component 1) ELISA Kit
别名PIEZO1; FAM38A; KIAA0233

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.049 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q92508

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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