一步法人血管内皮生长因子A(VEGFA)酶联免疫吸附检测试剂盒
- 货号：ELK033ES
- 价格：￥2,400.00 元
- 点击：2020
产品介绍
产品信息
| 英文名称 | EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit |
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| 别名 | VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor |
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规格与价格
| 48T 价格 | 1680 |
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| 96T 价格 | 2400 |
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| 96T*5 价格 | 10200 |
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检测参数
| 种属 | Human |
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| 检测类型 | Sandwich |
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| 灵敏度 | 31 pg/mL |
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| 检测范围 | 78.2-5000 pg/mL |
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| 样本类型 | Serum, plasma |
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| 实验时长 | 1.5h |
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研究信息
| 研究领域 | Cytokine;Apoptosis;Tumor immunity; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt编号 | P15692 |
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标准曲线
| 标准曲线图 |  |
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