人E3泛素蛋白连接酶HECTD3(HECTD3)酶联免疫吸附检测试剂盒

英文名称	Human HECTD3(E3 Ubiquitin-Protein Ligase HECTD3) ELISA Kit
别名	HECT domain-containing protein 3; HECT-type E3 ubiquitin transferase HECTD3

种属	Human
检测类型	Sandwich
灵敏度	0.065 ng/mL
检测范围	0.16-10 ng/mL
样本类型	serum, plasma and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HECTD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HECTD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HECTD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HECTD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q5T447

标准曲线图

【仅供科学研究使用】

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