人甲硫氨酸合成酶(MTR)酶联免疫吸附检测试剂盒

英文名称	Human MTR(Methionine synthase) ELISA Kit
别名	Methionine synthase; EC 2.1.1.13; 5-methyltetrahydrofolate--homocysteine methyltransferase; Vitamin-B12 dependent methionine synthase; MS

种属	Human
检测类型	Sandwich
灵敏度	0.051 ng/mL
检测范围	0.16-10 ng/mL
样本类型	serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MTR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MTR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q99707

标准曲线图

【仅供科学研究使用】

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