8异前列腺素F2α(8-iso-PGF2a)酶联免疫吸附检测试剂盒
- 货号：ELK0151
- 价格：￥2,800.00 元
- 点击：1092
产品介绍
产品信息
| 英文名称 | 8-iso-PGF2α(8-isoprostane) ELISA Kit |
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| 别名 | 8-isoprostane; 8-iso-PGF2α |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | General |
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| 检测类型 | Competitive Inhibition |
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| 灵敏度 | 4.47 pg/mL |
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| 检测范围 | 15.63-1000 pg/mL |
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| 样本类型 | serum, plasma and other biological fluids |
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| 实验时长 | 2.5h |
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研究信息
| 研究领域 | Endocrinology;Pulmonology;Hepatology;Hormone metabolism; |
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| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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