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小鼠磷脂酰肌醇4,5-二磷酸3-激酶催化亚基α(PIK3Ca)酶联免疫吸附检测试剂盒

  • 货号:ELK0043
  • 价格:¥2,600.00 元
  • 点击:2559

产品介绍

产品信息

英文名称Mouse PIK3Ca(Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide) ELISA Kit
别名phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; CCM4; CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; PI3K-alpha; p110-alpha

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.16 ng/mL
检测范围0.79-50 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIK3Ca. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIK3Ca. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIK3Ca, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIK3Ca in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P42337

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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