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马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒

  • 货号:ELK0022
  • 价格:¥2,800.00 元
  • 点击:557

产品介绍

产品信息

英文名称Horse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
别名TBARS; Thiobarbituric Acid Reactive Substance

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Horse
检测类型Competitive Inhibition
灵敏度3.55 nmol/mL
检测范围6.25-400 nmol/mL
样本类型Serum, plasma, cell lysates, cell culture supernates and other biological fluids
实验时长2h

研究信息

研究领域Metabolic pathway;Hepatology;Hormone metabolism;
检测原理This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

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【仅供科学研究使用】

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