Recombinant Human SUMO2 (N-6His)
- 货号：EPT277
- 价格：￥120.00 元
- 点击：1095
产品介绍
物理性质
| 分子量 | 13 KDa |
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| 表观分子量 | 17 KDa, reducing conditions |
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| 形态 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
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| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
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活性与内毒素
| 内毒素水平 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
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储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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| 运输条件 | The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
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详细说明
| 背景说明 | Small Ubiquitin-Related Modifier 2 (SUMO2) is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptidebond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. |
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