Recombinant E.coli Trp synthase (N-6His)
- 货号：EPT234
- 价格：￥3,780.00 元
- 点击：883
产品介绍
物理性质
| 分子量 | 28.7&43.8 KDa |
|---|
| 表观分子量 | 28&40-50 KDa, reducing conditions |
|---|
| 形态 | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. |
|---|
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
|---|
活性与内毒素
| 内毒素水平 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
|---|
储存与运输
| 储存方法 | Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles. |
|---|
| 运输条件 | The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below. |
|---|
详细说明
| 背景说明 | Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target. |
|---|
