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Recombinant Human IFN gamma (E. coli)

  • 货号:EPT184
  • 价格:¥1,200.00 元
  • 点击:1377

产品介绍

基本信息

规格50ug
表达宿主E.coli
标签E. coli

物理性质

分子量16.88 KDa
表观分子量16 KDa, reducing conditions
形态Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Mannitol, 0.1% Tween80, 5% Trehalose, pH6.0.
纯度Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

内毒素水平Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
活性Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml.

储存与运输

溶解方法Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
储存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
运输条件The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.

详细说明

背景说明IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.
【仅供科学研究使用】

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