Recombinant Human IFN gamma (E. coli)
- 货号：EPT184
- 价格：￥1,200.00 元
- 点击：1474
产品介绍
基本信息
| 规格 | 50ug |
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| 表达宿主 | E.coli |
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| 标签 | E. coli |
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物理性质
| 分子量 | 16.88 KDa |
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| 表观分子量 | 16 KDa, reducing conditions |
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| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Mannitol, 0.1% Tween80, 5% Trehalose, pH6.0. |
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| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
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活性与内毒素
| 内毒素水平 | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
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| 活性 | Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml. |
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储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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| 运输条件 | The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
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详细说明
| 背景说明 | IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF. |
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