Recombinant Human IL-6
- 货号：EPT155
- 价格：￥4,200.00 元
- 点击：635
产品介绍
基本信息
| 规格 | 50ug |
|---|---|
| 表达宿主 | E.coli |
| 标签 | NA |
物理性质
| 分子量 | 20.9 KDa |
|---|---|
| 表观分子量 | 20 KDa, reducing conditions |
| 形态 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
活性与内毒素
| 内毒素水平 | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
|---|---|
| 活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 20-100 pg/ml. |
储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
|---|---|
| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
详细说明
| 背景说明 | Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. |
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