Recombinant Human IL-2 (aldesleukin)
- 货号：EPT147
- 价格：￥600.00 元
- 点击：1269
产品介绍
基本信息
| 规格 | 10ug |
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| 表达宿主 | E.coli |
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| 标签 | aldesleukin |
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物理性质
| 分子量 | 15.5 KDa |
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| 表观分子量 | 14 KDa, reducing conditions |
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| 形态 | Lyophilized from a 0.2 μm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5. |
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| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
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活性与内毒素
| 内毒素水平 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
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| 活性 | Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml. |
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储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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| 运输条件 | The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
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详细说明
| 背景说明 | Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-145 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: it is not glycosylated; the molecule has serine substituted for cysteine at amino acid position 145; the aggregation state of molecule is likely to be different from that of native IL-2. |
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