Recombinant 2019-nCoV Papain-Like Protease
- 货号：EPT114
- 价格：￥5,400.00 元
- 点击：1373
产品介绍
物理性质
| 分子量 | 35.8 KDa |
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| 表观分子量 | 34 KDa, reducing conditions |
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| 形态 | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5. |
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| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
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活性与内毒素
| 活性 | Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol/min/μg. |
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储存与运输
| 储存方法 | Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles. |
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| 运输条件 | The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below. |
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详细说明
| 背景说明 | Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons. |
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