Recombinant 2019-nCoV Papain-Like Protease

产品介绍

{"gui_ge":"50ug","biao_da_su_zhu":"E.coli","biao_qian":"NA","fen_zi_liang":"35.8 KDa","biao_guan_fen_zi_liang":"34 KDa, reducing conditions","xing_tai":"Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.","chun_du":"Greater than 95% as determined by reducing SDS-PAGE.","huo_xing":"Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol\/min\/μg.","chu_cun_fang_fa":"Store at ≤-70°C, stable for 6 months after receipt.\nStore at ≤-70°C, stable for 3 months under sterile conditions after opening. \nPlease minimize freeze-thaw cycles. ","yun_shu_tiao_jian":"The product is shipped on dry ice\/polar packs.\nUpon receipt, store it immediately at the temperature listed below.","bei_jing_shuo_ming":"Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons."}