Recombinant Human NovoNectin
- 货号：EPT094
- 价格：￥960.00 元
- 点击：1565
产品介绍
物理性质
| 分子量 | 62.7 KDa |
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| 表观分子量 | 60-80 KDa, reducing conditions |
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| 形态 | Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 5.5. |
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| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
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活性与内毒素
| 内毒素水平 | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
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| 活性 | Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml. |
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储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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| 运输条件 | The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
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详细说明
| 背景说明 | Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result. |
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