Recombinant Human IL-33
- 货号：EPT054
- 价格：￥1,800.00 元
- 点击：746
产品介绍
基本信息
| 规格 | 10ug |
|---|---|
| 表达宿主 | E.coli |
| 标签 | NA |
物理性质
| 分子量 | 18.1 KDa |
|---|---|
| 表观分子量 | 18 KDa, reducing conditions |
| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
活性与内毒素
| 内毒素水平 | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
|---|---|
| 活性 | Immobilized Mouse ST2-His(Cat#CK89) at 5μg/ml (100 μl/well) can bind Human IL-33(Cat#C091).The ED50 of Human IL-33(Cat#C091) is 0.63 ug/ml. |
储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
|---|---|
| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
详细说明
| 背景说明 | Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length. |
|---|
相关产品:
| 产品名称 | 点击数 |
|---|---|
| 罗丹明B-BSA（或OVA）抗原 | 4125 |
| 金刚烷胺-BSA（或OVA）抗原 | 3838 |
| 利巴韦林-BSA（或OVA）抗原 | 3981 |
| 甲硝唑-BSA（或OVA）抗原 | 4167 |
| 林可霉素-BSA（或OVA）抗原 | 4087 |
| 尼卡巴嗪代谢物（DNC）-BSA（或OVA）抗原 | 3895 |
| 氯霉素(CAP)-BSA（或OVA）抗原 | 4138 |
| 氟苯尼考-BSA（或OVA）抗原 | 4223 |
| 过氧化物酶标氯霉素抗原（HRP-CAP-BSA和HRP-CAP-OVA） | 4268 |
| 小鼠抗乙肝表面抗原 | 4069 |
| 小鼠抗乙肝e抗原 | 3872 |
| 小鼠抗乙肝核心抗原 | 3945 |
| 小鼠抗人BSA | 4078 |
| HRP-小鼠抗乙肝核心抗原 | 4165 |
| HRP-小鼠抗乙肝e抗原 | 4116 |
| HRP-小鼠抗乙肝表面抗原 | 4211 |
| FITC-小鼠抗乙肝核心抗原 | 4254 |
| FITC-小鼠抗乙肝e抗原 | 4007 |
| FITC-小鼠抗乙肝表面抗原 | 4001 |
| BIOTIN-小鼠抗乙肝核心抗原 | 4130 |