Recombinant Human TNF alpha
- 货号：EPT038
- 价格：￥1,440.00 元
- 点击：1256
产品介绍
基本信息
| 规格 | 50ug |
|---|---|
| 表达宿主 | E.coli |
| 标签 | NA |
物理性质
| 分子量 | 17.5 KDa |
|---|---|
| 表观分子量 | 16 KDa, reducing conditions |
| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0. |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
活性与内毒素
| 内毒素水平 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
|---|---|
| 活性 | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml. |
储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
详细说明
| 背景说明 | TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease. |
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