鸡血管内皮生长因子A(VEGFA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Chicken VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit","alternative_names":"VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor","species":"Chicken","assay_type":"Sandwich","sensitivity":"6.7 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma and other biological fluids.","assay_length":"3.5h","research_area":"Cytokine;Apoptosis;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P67964","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2037-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2037-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2037",}